Relationship between TLR4 and MCP2 expression levels and habitual abortion.
نویسندگان
چکیده
Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression in peripheral blood and placental villi of patients and healthy fertile women. Based on our previous research, 85 subjects with habitual abortion (study group) and 40 healthy fertile women (control group), who were admitted to our hospital between June 2013 and December 2014, were enrolled in this study. After these subjects signed written informed consent, peripheral blood samples and villous tissues were collected, from which the total RNA was extracted. The expression of TLR4 and MCP2 was detected with quantitative reverse transcription-polymerase chain reaction, using GAPDH as a reference control. The expression of TLR4 and MCP2 in the peripheral blood and villous tissues of the study group was significantly higher than that of the control group (P < 0.05). A positive correlation was also observed between the changes in expression levels of TLR4 and MCP2. In conclusion, TLR4 and MCP2 expression correlated with the occurrence of habitual abortion. Detecting expression changes in TLR4 and MCP2 in the peripheral blood is a feasible method for predicting the occurrence of abortion in women of child-bearing age.
منابع مشابه
Studies on the Different Methods for Diagnosis and Identification of Listeria Monocytogenes and its Relationship to Habitual Abortion
SUMMARY A bacteriologic study has been done over 150 placenta (or the remaining of curettage) and vaginal sample in women inflicted with abortion in Kerman. A long with these tests, serologic tests with indirect immunotluorescence were carried out to determine the amount of serum antibodies. These tests were compared with similar samples derived from 70 normal childbirth cases. Listeria wa...
متن کاملThe Effect of TLR4 Asp299Gly polymorphism on IL-6 and IL -18 expression in H.pylori infected patients
Background & Aims: Helicobacter pylori (H. pylori) infection is associated with peptic ulcer and gastric cancer. Polymorphisms of the genes coding Toll-like receptors (TLRs) may influence the innate and adaptive immune responses and affect the susceptibility to H. pylori or the disease outcomes. But the details and association to different polymorphis...
متن کاملRelationship between TLR4 and CCL2 expression and recurrent spontaneous abortion.
The increasing incidence of recurrent spontaneous abortion (RSA) severely affects women's health. The involvement of the immune system during pregnancy and its contribution to RSA draw researchers' attention. Both Toll-like receptor 4 (TLR4) and chemokine (C-C motif) ligand 2 (CCL2) have been linked to various pregnancy disorders. In this study, we quantified TLR4 and CCL2 levels in a case-cont...
متن کاملThe Relationship between Homocysteine Levels and Spontaneous Abortion in Iranian Women with Migraine
متن کامل
The incidence of spontaneous abortion in mothers with blood group O compared with other blood types
Although ABO incompatibility between mother and fetus has long been suspected as cause of spontaneous abortion in man, its precise contribution has not been completely resolved. In spite of reports in which the incompatible mating was recognized to be a cause of habitual abortion, and which eventually results in infertility or a reduction in the number of living children compared with the numbe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Genetics and molecular research : GMR
دوره 15 2 شماره
صفحات -
تاریخ انتشار 2016